Rnase A Stability Room Temperature

Storage at room temperature does not affect enzyme performance all our products have been tested for stability at room temperature for an extended time.

Rnase a stability room temperature. Total rna stability at room temperature 25 c. Rnases in general are rich in disulfide bounds which grant them extra stability in adverse conditions such as heat. Nuclease stability of rna synthesized with the rt pcr competitor construction kit. The ph value is equilibrated by adding 0 1 fold the volume of 1 m tris cl ph 7 4.

Rnase a at the concentrations indicated was incubated at room temperature for 1 hr with radiolabeled standard rna or rna synthesized by the rt pcr competitor construction kit in 1 transcription buffer. Stranded rna 1 room temperature. Rnase a is a single chain polypeptide containing 4 disulfide bridges. In contrast to rnase b it is not a glycoprotein 2 rnase a can be inhibited by alkylation of his12 4 or his119 which are present in the active site of the enzyme 3 activators of rnase a include potassium and sodium salts.

At 100 c an rnase a solution is most stable between ph 2 0 and 4 5 13 for removal of rna from preparations of plasmid dna. However 20ºc long term storage is recommended for both l yophilized and pre mixed solutions of rnase a. That s why some rna extraction protocols include 2 mercaptoethanol in its. Storage stability this product remains active for at least 2 years when stored properly at 20 c.

Inactivation of dnase activity. Rnase a can be dissolved at a concentration of 1 to 10 mg ml in 10 mm tris hcl ph 7 5 15 mm nacl heated to 100 c for 15 minutes to inactivate contaminating dnases and cooled slowly to room temperature and dispense into aliquots. Both the excitation and emission slit widths were fixed at 5 nm. The fluorescence experiments were done at room temperature 23 c with a shimadzu rf 5000 spectrofluorimeter.

Rnase a premixed solutions retain enzymatic activity at room temperature or 2 8ºc for extended periods. How can i improve rna quality with low 260 230 ratio. For illustration the qpcr performance of a product that was stored at 20 c was compared to the same product stored at room temperature for 4 weeks. I understand the role of rnase and most of us take the optimum measure to get rid it.